ESIC Hospital, Basaidarapur
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Head of Department: Dr. Sanjay Singhal, MBBS, MD( Microbiology)     

Departmental Staff includes- Professor (2), Assistant Professors (2), Senior Residents (2), Laboratory Technicians, Laboratory Attendants and Nursing Orderlies


Education:                       MBBS(1985) from KG Medical College, Lucknow

                                        MD (1989) from KG Medical College, Lucknow


Teaching Experience:      Under graduate teaching:              32 years

                                        Post Graduate Teaching:                27 years


Teaching staff:


Sr. No.






Dr. Sanjay Singhal

Director Professor & HOD



Dr. Sonia Khattar




Dr. Sangita Gupta

Assistant Professor cum NFSG



Dr. Devki Verma

Assistant Professor


Services Provided: The Department functions as a Clinical Microbiology Laboratory with a focus on the clinical and lab diagnosis of infectious diseases and has been upgraded for teaching and research activities. All OPD samples for microbiological investigations are received up to 1:00 p.m. on all working days and emergency samples are accepted 24 hours. The Department receives approximately 9000-10000 samples and performs 18,000-20,000 investigations every month.

Department has received permission from MCI to start MD microbiology course (2 seats). Department has a well equipped library with books and  journals. It has a museum with over  specimens, charts. It has a seven head teaching microscope with computer projection and photography attachment  and has more than 200 slides pertaining to various infections.

Department along with the hospital is ISO certified. It has been participating in EQAS for bacteriology and serology run by IAMM, EQAS VDRL conducted by VMMC and EQAS Mycology program by PGI Chandigarh.

It has been giving training to students pursuing PhD, MSc (Microbiology), BSc (Microbiology), BMLT, DMLT and biotechnology courses.

Department also supervises hospital biomedical waste management and hospital infection control and gives training to hospital staff for this.


Tests Done in the Department of Microbiology


Microbiology Lab has the following sections:

  1. Bacteriology

  2. Mycology

  3. Mycobacteriology

  4. Parasitology

  5. Infection Control

  6. Serology

  7. Immunology

  8. Virology

  9. Molecular Biology


Sub –Bacteriology (for all samples)

    1. Gram’s Stain of all clinical samples

    2. Conventional Culture and Sensitivity testing (Aerobic & Anaerobic)

    3. Automated Culture for blood and body fluids (Aerobic & Anaerobic)

    4. Automated Identification and Susceptibility testing by ViTek 2

    5. Albert’s Stain and culture for C. diphtheriae

    6. Stool Hanging Drop preparation and culture for V. cholerae along with serotyping

    7. Vaginal Smear (Wet Prep), Gram stain, and endocervical swab culture

    8.     Stool Hanging Drop and Culture for Vibrio cholerae with serotyping

    9. Stool culture for Salmonella, Shigella, and other pathogens

    10. Latex Agglutination test for meningitis-causing bacteria

    11. CSF culture for Meningitis


Sub–Mycology (for all samples)

    1. KOH Preparation and  staining for fungal pathogens

    2. INDIA INK Preparation

    3. Fungal Culture

    4. AFST for yeasts (by disc diffusion and MIC)


Sub –Mycobacteriology (AFB)    (All samples)

    1. Direct smear for AFB (ZN Stain)

    2. Mycobacterial Culture (Automated and Conventional)

    3. Culture and susceptibility for Non-tuberculous Mycobacteria

    4. Slit Smear for Lepra Bacilli

    5. Others-( HAIN Line probe assay for molecular drug sensitivity for INH and Rifampicin – only for suspected MDR TB cases from sputum samples and positive culture isolates)

    6. CBNAAT (By Truenat) for M. tuberculosis


Sub – Parasitology

    1. Stool Routine Microscopy

    2. Stool PH

    3. Stool Reducing Sugar

    4. Stool Occult Blood

    5. Stool- Modified ZN staining for Cryptosporidium and other parasites

    6. Malaria antigen detection test

    7. Peripheral Blood Smear for parasites- Malaria, Microfilaria & others

    8. Kala azar Antibody detection rapid card test ( IG M/ IG G)

    9. LD bodies in Bone marrow smears

    10. QBC for malaria



Sub–Hospital Infection


    1. Water Culture

    2. Disinfectant testing

    3. Biological indicators testing

    4. Carrier Studies

    5. Environmental Cultures in case of Outbreaks


Sub –  Serology, Immunology, and Virology


    1. Widal

    2. Typhidot

    3. ASO

    4. CRP

    5. VDRL( from Serum  and CSF)/ RPR

    6. ELISA for Syphillis



    9. TORCH-Rubella IgG

    10. TORCH-Rubella IgM

    11. TORCH-CMV IgG

    12. TORCH-CMV IgM

    13. TORCH-Herpes I IgG

    14. TORCH-Herpes II IgG

    15. TORCH-Herpes I IgM

    16. TORCH-Herpes II IgM

    17. Dengue IgG/IgM (card test)

    18. Dengue IgM (Elisa)

    19. Dengue NS1 Antigen ELISA

    20. Chikungunya ELISA

    21. HBs Ag Rapid card test

    22. Anti HCV Rapid card Test

    23. HBs Antigen ELISA

    24. Anti HBS Titres

    25. Anti HBc Total ELISA

    26. Anti HBc IgM ELISA



Sub –  Immunology


    1.      ANA/ANF ELISA

    2.      ANA by IIFA using HEP2 and primate liver cell line

    3. ds DNA ELISA

    4. ds DNA by IIFA using Crithidea lucilae

    5. ASMA (Anti Smooth muscle antibody) , LKM, AMA,  using IIFA

    6. AMA (Anti Mitochondrial antibody)  using ELISA

    7. Anti PR3 ELISA

    8. Anti MPO ELISA

    9. ANCA screening using  IIFA

    10. ENA profile for Antinuclear antibodies using LIA

    11. ENA profile for Anticytoplasmic antibodies using LIA

    12. ENA profile for Vasculitis using LIA

    13. ENA profile for autoimmune liver diseases using LIA

    14. ENA profile for Myositis using LIA

    15. RA

    16. Total IgE

  1.  Virology

    1. HIV I and II ELISA

    2. HIV I and II Rapid Test

    3. Western Blot LIA

  2. Molecular Biology

    1. RTPCR (Truenat based) for SARS CoV2

    2. rRTPCR (open system) soon to begin



Last updated / Reviewed : 2022-08-04



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