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Microbiology

DEPARTMENT OF MICROBIOLOGY

 

 

Head of Department: Dr. Sanjay Singhal, MBBS, MD( Microbiology)     

Departmental Staff includes- Professor (2), Assistant Professors (2), Senior Residents (2), Laboratory Technicians, Laboratory Attendants and Nursing Orderlies

 

Education:                       MBBS(1985) from KG Medical College, Lucknow

                                        MD (1989) from KG Medical College, Lucknow

 

Teaching Experience:      Under graduate teaching:              32 years

                                        Post Graduate Teaching:                27 years

 

Teaching staff:

 

Sr. No.	Name	Designation	phone	E-mail
1	Dr. Sanjay Singhal	Director Professor & HOD	8447211351	dr.sanjay.singhal@esic.nic.in drsanjaysinghal@gmail.com
2	Dr. Sonia Khattar	Professor	9810692484	dr.sonia.khattar@esic.nic.in maliksonia@yahoo.com
3	Dr. Sangita Gupta	Assistant Professor cum NFSG	01125970839	dr.sangita.gupta@esic.nic.in
4	Dr. Devki Verma	Assistant Professor	9810905606	devkiverma1908@gmail.com

Services Provided: The Department functions as a Clinical Microbiology Laboratory with a focus on the clinical and lab diagnosis of infectious diseases and has been upgraded for teaching and research activities. All OPD samples for microbiological investigations are received up to 1:00 p.m. on all working days and emergency samples are accepted 24 hours. The Department receives approximately 9000-10000 samples and performs 18,000-20,000 investigations every month.

Department has received permission from MCI to start MD microbiology course (2 seats). Department has a well equipped library with books and  journals. It has a museum with over  specimens, charts. It has a seven head teaching microscope with computer projection and photography attachment  and has more than 200 slides pertaining to various infections.

Department along with the hospital is ISO certified. It has been participating in EQAS for bacteriology and serology run by IAMM, EQAS VDRL conducted by VMMC and EQAS Mycology program by PGI Chandigarh.

It has been giving training to students pursuing PhD, MSc (Microbiology), BSc (Microbiology), BMLT, DMLT and biotechnology courses.

Department also supervises hospital biomedical waste management and hospital infection control and gives training to hospital staff for this.

 

Tests Done in the Department of Microbiology

 

Microbiology Lab has the following sections:

1. Bacteriology

2. Mycology

3. Mycobacteriology

4. Parasitology

5. Infection Control

6. Serology

7. Immunology

8. Virology

9. Molecular Biology

 

Sub –Bacteriology (for all samples)

1. 1. Gram’s Stain of all clinical samples

   2. Conventional Culture and Sensitivity testing (Aerobic & Anaerobic)

   3. Automated Culture for blood and body fluids (Aerobic & Anaerobic)

   4. Automated Identification and Susceptibility testing by ViTek 2

   5. Albert’s Stain and culture for C. diphtheriae

   6. Stool Hanging Drop preparation and culture for V. cholerae along with serotyping

   7. Vaginal Smear (Wet Prep), Gram stain, and endocervical swab culture

   8.     Stool Hanging Drop and Culture for Vibrio cholerae with serotyping

   9. Stool culture for Salmonella, Shigella, and other pathogens

   10. Latex Agglutination test for meningitis-causing bacteria

   11. CSF culture for Meningitis

 

Sub–Mycology (for all samples)

1. 1. KOH Preparation and  staining for fungal pathogens

   2. INDIA INK Preparation

   3. Fungal Culture

   4. AFST for yeasts (by disc diffusion and MIC)

 

Sub –Mycobacteriology (AFB)    (All samples)

1. 1. Direct smear for AFB (ZN Stain)

   2. Mycobacterial Culture (Automated and Conventional)

   3. Culture and susceptibility for Non-tuberculous Mycobacteria

   4. Slit Smear for Lepra Bacilli

   5. Others-( HAIN Line probe assay for molecular drug sensitivity for INH and Rifampicin – only for suspected MDR TB cases from sputum samples and positive culture isolates)

   6. CBNAAT (By Truenat) for M. tuberculosis

 

Sub – Parasitology

1. 1. Stool Routine Microscopy

   2. Stool PH

   3. Stool Reducing Sugar

   4. Stool Occult Blood

   5. Stool- Modified ZN staining for Cryptosporidium and other parasites

   6. Malaria antigen detection test

   7. Peripheral Blood Smear for parasites- Malaria, Microfilaria & others

   8. Kala azar Antibody detection rapid card test ( IG M/ IG G)

   9. LD bodies in Bone marrow smears

   10. QBC for malaria

 

 

Sub–Hospital Infection

 

1. 1. Water Culture

   2. Disinfectant testing

   3. Biological indicators testing

   4. Carrier Studies

   5. Environmental Cultures in case of Outbreaks

 

Sub –  Serology, Immunology, and Virology

 

1. 1. Widal

   2. Typhidot

   3. ASO

   4. CRP

   5. VDRL( from Serum  and CSF)/ RPR

   6. ELISA for Syphillis

   7. TORCH-TOXO IgG

   8. TORCH-TOXO IgM

   9. TORCH-Rubella IgG

   10. TORCH-Rubella IgM

   11. TORCH-CMV IgG

   12. TORCH-CMV IgM

   13. TORCH-Herpes I IgG

   14. TORCH-Herpes II IgG

   15. TORCH-Herpes I IgM

   16. TORCH-Herpes II IgM

   17. Dengue IgG/IgM (card test)

   18. Dengue IgM (Elisa)

   19. Dengue NS1 Antigen ELISA

   20. Chikungunya ELISA

   21. HBs Ag Rapid card test

   22. Anti HCV Rapid card Test

   23. HBs Antigen ELISA

   24. Anti HBS Titres

   25. Anti HBc Total ELISA

   26. Anti HBc IgM ELISA

 

 

Sub –  Immunology

 

1. 1.      ANA/ANF ELISA

   2.      ANA by IIFA using HEP2 and primate liver cell line

   3. ds DNA ELISA

   4. ds DNA by IIFA using Crithidea lucilae

   5. ASMA (Anti Smooth muscle antibody) , LKM, AMA,  using IIFA

   6. AMA (Anti Mitochondrial antibody)  using ELISA

   7. Anti PR3 ELISA

   8. Anti MPO ELISA

   9. ANCA screening using  IIFA

   10. ENA profile for Antinuclear antibodies using LIA

   11. ENA profile for Anticytoplasmic antibodies using LIA

   12. ENA profile for Vasculitis using LIA

   13. ENA profile for autoimmune liver diseases using LIA

   14. ENA profile for Myositis using LIA

   15. RA

   16. Total IgE

2.  Virology

3. 1. HIV I and II ELISA

   2. HIV I and II Rapid Test

   3. Western Blot LIA

4. Molecular Biology

   1. RTPCR (Truenat based) for SARS CoV2

   2. rRTPCR (open system) soon to begin

 

 

Last updated / Reviewed : 2022-08-04